Putative effector genes distinguish two pathogenicity groups of Fusarium oxysporum f. sp. spinaciae.
Identifieur interne : 000070 ( Main/Exploration ); précédent : 000069; suivant : 000071Putative effector genes distinguish two pathogenicity groups of Fusarium oxysporum f. sp. spinaciae.
Auteurs : Alexander Marcus Batson [États-Unis] ; Like Fokkens [Pays-Bas] ; Martijn Rep [Pays-Bas] ; Lindsey Du Toit [États-Unis]Source :
- Molecular plant-microbe interactions : MPMI [ 0894-0282 ] ; 2020.
Abstract
Fusarium wilt of spinach, caused by Fusarium oxysporum f. sp. spinaciae (Fos), is an important disease during warm conditions in production regions with acid soils, yet little is known about what confers pathogenicity to spinach in Fos genetically. To identify candidate fungal genes that contribute to spinach Fusarium wilt, each of 69 geographically diverse F. oxysporum isolates was tested for pathogenicity on each of three spinach inbreds. Thirty-nine isolates identified as Fos caused quantitative differences in disease severity among the inbreds that revealed two distinct pathogenicity groups of Fos. Putative effector gene profiles, predicted from whole-genome sequences generated for nine Fos isolates and five non-pathogenic, spinach-associated F. oxysporum (NPS) isolates, distinguished the Fos isolates from the NPS isolates, and separated the Fos isolates into two groups. Five of the putative effector genes appeared to be unique to Fos as they were not found in 222 other publicly available genome assemblies of F. oxysporum, implicating potential involvement of these genes in pathogenicity to spinach. In addition, two combinations of the 14 known Secreted in Xylem (SIX) genes that have been affiliated with host pathogenicity in other formae speciales of F. oxysporum were identified in genome assemblies of the nine Fos isolates: either SIX8 and SIX9, or SIX4, SIX8, and SIX14. Characterization of these putative effector genes should aid in understanding mechanisms of pathogenicity in Fos, developing molecular tools for rapid detection and quantification of Fos, and breeding for resistance to Fusarium wilt in spinach.
DOI: 10.1094/MPMI-06-20-0145-R
PubMed: 33103963
Affiliations:
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L.Fokkens@uva.nl.</nlm:affiliation><country wicri:rule="url">Pays-Bas</country><wicri:regionArea>University of Amsterdam Swammerdam Institute for Life Sciences, 215691, Plant pathology, Amsterdam, North Holland</wicri:regionArea><wicri:noRegion>North Holland</wicri:noRegion></affiliation></author><author><name sortKey="Rep, Martijn" sort="Rep, Martijn" uniqKey="Rep M" first="Martijn" last="Rep">Martijn Rep</name><affiliation wicri:level="1"><nlm:affiliation>University of Amsterdam Swammerdam Institute for Life Sciences, 215691, Plant pathology, Amsterdam, North Holland, Netherlands; m.rep@uva.nl.</nlm:affiliation><country wicri:rule="url">Pays-Bas</country><wicri:regionArea>University of Amsterdam Swammerdam Institute for Life Sciences, 215691, Plant pathology, Amsterdam, North Holland</wicri:regionArea><wicri:noRegion>North Holland</wicri:noRegion></affiliation></author><author><name sortKey="Du Toit, Lindsey" sort="Du Toit, Lindsey" uniqKey="Du Toit L" first="Lindsey" last="Du Toit">Lindsey Du Toit</name><affiliation wicri:level="1"><nlm:affiliation>Washington State University, 6760, Plant Pathology, WSU Mount Vernon NWREC, 16650 State Route 536, Mount Vernon, Washington, United States, 98273-4768; 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alex.batson@wsu.edu.</nlm:affiliation><country wicri:rule="url">États-Unis</country><wicri:regionArea>Washington State University, 6760, Plant Pathology, WSU Mount Vernon NWREC, Pullman, Washington</wicri:regionArea><placeName><region type="state">Washington (État)</region></placeName></affiliation></author><author><name sortKey="Fokkens, Like" sort="Fokkens, Like" uniqKey="Fokkens L" first="Like" last="Fokkens">Like Fokkens</name><affiliation wicri:level="1"><nlm:affiliation>University of Amsterdam Swammerdam Institute for Life Sciences, 215691, Plant pathology, Amsterdam, North Holland, Netherlands; L.Fokkens@uva.nl.</nlm:affiliation><country wicri:rule="url">Pays-Bas</country><wicri:regionArea>University of Amsterdam Swammerdam Institute for Life Sciences, 215691, Plant pathology, Amsterdam, North Holland</wicri:regionArea><wicri:noRegion>North Holland</wicri:noRegion></affiliation></author><author><name sortKey="Rep, Martijn" sort="Rep, Martijn" uniqKey="Rep M" first="Martijn" last="Rep">Martijn Rep</name><affiliation wicri:level="1"><nlm:affiliation>University of Amsterdam Swammerdam Institute for Life Sciences, 215691, Plant pathology, Amsterdam, North Holland, Netherlands; m.rep@uva.nl.</nlm:affiliation><country wicri:rule="url">Pays-Bas</country><wicri:regionArea>University of Amsterdam Swammerdam Institute for Life Sciences, 215691, Plant pathology, Amsterdam, North Holland</wicri:regionArea><wicri:noRegion>North Holland</wicri:noRegion></affiliation></author><author><name sortKey="Du Toit, Lindsey" sort="Du Toit, Lindsey" uniqKey="Du Toit L" first="Lindsey" last="Du Toit">Lindsey Du Toit</name><affiliation wicri:level="1"><nlm:affiliation>Washington State University, 6760, Plant Pathology, WSU Mount Vernon NWREC, 16650 State Route 536, Mount Vernon, Washington, United States, 98273-4768; dutoit@wsu.edu.</nlm:affiliation><country wicri:rule="url">États-Unis</country><wicri:regionArea>Washington State University, 6760, Plant Pathology, WSU Mount Vernon NWREC, 16650 State Route 536, Mount Vernon, Washington, United States</wicri:regionArea><wicri:noRegion>United States</wicri:noRegion></affiliation></author></analytic><series><title level="j">Molecular plant-microbe interactions : MPMI</title><idno type="ISSN">0894-0282</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Fusarium wilt of spinach, caused by <i>Fusarium oxysporum</i> f. sp. <i>spinaciae</i> (<i>Fos</i>), is an important disease during warm conditions in production regions with acid soils, yet little is known about what confers pathogenicity to spinach in <i>Fos</i> genetically. To identify candidate fungal genes that contribute to spinach Fusarium wilt, each of 69 geographically diverse <i>F. oxysporum</i> isolates was tested for pathogenicity on each of three spinach inbreds. Thirty-nine isolates identified as <i>Fos</i> caused quantitative differences in disease severity among the inbreds that revealed two distinct pathogenicity groups of <i>Fos</i>. Putative effector gene profiles, predicted from whole-genome sequences generated for nine <i>Fos</i> isolates and five non-pathogenic, spinach-associated <i>F. oxysporum</i> (NPS) isolates, distinguished the <i>Fos</i> isolates from the NPS isolates, and separated the <i>Fos</i> isolates into two groups. Five of the putative effector genes appeared to be unique to <i>Fos</i> as they were not found in 222 other publicly available genome assemblies of <i>F. oxysporum</i>, implicating potential involvement of these genes in pathogenicity to spinach. In addition, two combinations of the 14 known <i>Secreted in Xylem</i> (<i>SIX</i>) genes that have been affiliated with host pathogenicity in other formae speciales of <i>F. oxysporum</i> were identified in genome assemblies of the nine <i>Fos</i> isolates: either <i>SIX8</i> and <i>SIX9</i>, or <i>SIX4</i>, <i>SIX8</i>, and <i>SIX14</i>. Characterization of these putative effector genes should aid in understanding mechanisms of pathogenicity in <i>Fos</i>, developing molecular tools for rapid detection and quantification of <i>Fos</i>, and breeding for resistance to Fusarium wilt in spinach.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">33103963</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>26</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0894-0282</ISSN><JournalIssue CitedMedium="Print"><PubDate><Year>2020</Year><Month>Oct</Month><Day>26</Day></PubDate></JournalIssue><Title>Molecular plant-microbe interactions : MPMI</Title><ISOAbbreviation>Mol Plant Microbe Interact</ISOAbbreviation></Journal><ArticleTitle>Putative effector genes distinguish two pathogenicity groups of <i>Fusarium oxysporum</i> f. sp. <i>spinaciae</i>.</ArticleTitle><ELocationID EIdType="doi" ValidYN="Y">10.1094/MPMI-06-20-0145-R</ELocationID><Abstract><AbstractText>Fusarium wilt of spinach, caused by <i>Fusarium oxysporum</i> f. sp. <i>spinaciae</i> (<i>Fos</i>), is an important disease during warm conditions in production regions with acid soils, yet little is known about what confers pathogenicity to spinach in <i>Fos</i> genetically. To identify candidate fungal genes that contribute to spinach Fusarium wilt, each of 69 geographically diverse <i>F. oxysporum</i> isolates was tested for pathogenicity on each of three spinach inbreds. Thirty-nine isolates identified as <i>Fos</i> caused quantitative differences in disease severity among the inbreds that revealed two distinct pathogenicity groups of <i>Fos</i>. Putative effector gene profiles, predicted from whole-genome sequences generated for nine <i>Fos</i> isolates and five non-pathogenic, spinach-associated <i>F. oxysporum</i> (NPS) isolates, distinguished the <i>Fos</i> isolates from the NPS isolates, and separated the <i>Fos</i> isolates into two groups. Five of the putative effector genes appeared to be unique to <i>Fos</i> as they were not found in 222 other publicly available genome assemblies of <i>F. oxysporum</i>, implicating potential involvement of these genes in pathogenicity to spinach. In addition, two combinations of the 14 known <i>Secreted in Xylem</i> (<i>SIX</i>) genes that have been affiliated with host pathogenicity in other formae speciales of <i>F. oxysporum</i> were identified in genome assemblies of the nine <i>Fos</i> isolates: either <i>SIX8</i> and <i>SIX9</i>, or <i>SIX4</i>, <i>SIX8</i>, and <i>SIX14</i>. Characterization of these putative effector genes should aid in understanding mechanisms of pathogenicity in <i>Fos</i>, developing molecular tools for rapid detection and quantification of <i>Fos</i>, and breeding for resistance to Fusarium wilt in spinach.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Batson</LastName><ForeName>Alexander Marcus</ForeName><Initials>AM</Initials><AffiliationInfo><Affiliation>Washington State University, 6760, Plant Pathology, WSU Mount Vernon NWREC, Pullman, Washington, United States; alex.batson@wsu.edu.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fokkens</LastName><ForeName>Like</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>University of Amsterdam Swammerdam Institute for Life Sciences, 215691, Plant pathology, Amsterdam, North Holland, Netherlands; L.Fokkens@uva.nl.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rep</LastName><ForeName>Martijn</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>University of Amsterdam Swammerdam Institute for Life Sciences, 215691, Plant pathology, Amsterdam, North Holland, Netherlands; m.rep@uva.nl.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>du Toit</LastName><ForeName>Lindsey</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Washington State University, 6760, Plant Pathology, WSU Mount Vernon NWREC, 16650 State Route 536, Mount Vernon, Washington, United States, 98273-4768; dutoit@wsu.edu.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>10</Month><Day>26</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Mol Plant Microbe Interact</MedlineTA><NlmUniqueID>9107902</NlmUniqueID><ISSNLinking>0894-0282</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>10</Month><Day>26</Day><Hour>12</Hour><Minute>9</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>10</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>10</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">33103963</ArticleId><ArticleId IdType="doi">10.1094/MPMI-06-20-0145-R</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Pays-Bas</li><li>États-Unis</li></country><region><li>Washington (État)</li></region></list><tree><country name="États-Unis"><region name="Washington (État)"><name sortKey="Batson, Alexander Marcus" sort="Batson, Alexander Marcus" uniqKey="Batson A" first="Alexander Marcus" last="Batson">Alexander Marcus Batson</name></region><name sortKey="Du Toit, Lindsey" sort="Du Toit, Lindsey" uniqKey="Du Toit L" first="Lindsey" last="Du Toit">Lindsey Du Toit</name></country><country name="Pays-Bas"><noRegion><name sortKey="Fokkens, Like" sort="Fokkens, Like" uniqKey="Fokkens L" first="Like" last="Fokkens">Like Fokkens</name></noRegion><name sortKey="Rep, Martijn" sort="Rep, Martijn" uniqKey="Rep M" first="Martijn" last="Rep">Martijn Rep</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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